Preprint sharing

Authors may share preprints, anywhere and at any time on a preprint servers e.g Research square, as this will not count as prior publication.

Studies in humans and animals

Authors must follow ethical guidelines for studies carried out in humans and animals.

Studies in humans

Work which involves the use of human subjects should be carried out in accordance with the World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects.

Manuscripts should follow the International Committee of Medical Journal Editors (ICMJE) recommendations for the conduct, reporting, editing and publication of scholarly work in medical journals and aim to be representative of human populations in terms of sex, age and ethnicity. Sex and gender terms should be used correctly, as outlined by WHO (World Health Organization).

Manuscripts must include a statement that all procedures were performed in compliance with relevant laws and institutional guidelines and have been approved by the appropriate institutional committee(s). The statement should contain the date and reference number of the ethical approval(s) obtained.

Manuscripts must also include a statement that the privacy rights of human subjects have been observed and that informed consent was obtained for experimentation with human subjects.

This journal will not accept manuscripts that contain data derived from unethically sourced organs or tissue, including from executed prisoners or prisoners of conscience, consistent with recommendations by Global Rights Compliance on Mitigating Human Rights Risks in Transplantation Medicine. For all studies that use human organs or tissues, sufficient evidence must be provided that these were procured in line with WHO Guiding Principles on Human Cell, Tissue and Organ Transplantation. The source of the organs or tissues used in clinical research must be transparent and traceable. If your manuscript describes organ transplantation you must additionally declare within the manuscript that:

• autonomous consent free from coercion was obtained from the donor(s) or their next of kin.
• organs and/or tissues were not sourced from executed prisoners or prisoners ofconscience.

Studies in animals

All animal experiments should comply with ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines.

Studies should be carried out in accordance with Guidance on the operation of the Animals (Scientific Procedures) Act 1986 and associated guidelines, EU Directive 2010/63 for the protection of animals used for scientific purposes or the NIH (National Research Council) Guide for the Care and Use of Laboratory Animals (PDF).

The sex of animals, and where appropriate, the influence (or association) of sex on the results of the study must be indicated and a statement included in your manuscript that such guidelines as listed above have been followed.